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Glycophorin A Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-2428

Novus Biologicals, part of Bio-Techne
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NB100-2428

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Peptide ELISA, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-SVEIENPETSDQ corresponding to C-Terminus according to NP_002090.2.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glycophorin A Antibody

Western Blot: Glycophorin A Antibody [NB100-2428]

Western Blot: Glycophorin A Antibody [NB100-2428]

Western Blot: Glycophorin A Antibody [NB100-2428] - Staining of Human Peripheral Blood Monocytes (A) and Kidney (B) lysate (35 ug protein in RIPA buffer). Antibody at 1 ug/mL. Detected by chemiluminescence.

Applications for Glycophorin A Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:2000

Western Blot

1 - 3 ug/mL
Application Notes
WB: Approx. 18 kDa band observed in human liver lysates (calculated MW of 16.4 kDa band according to NP_002090.2). Use in ICC/IF was reported in scientific literature (PMID: 28210226).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Glycophorin A

Glycophorins A (GPA) and B (GPB) are single pass membrane sialoglycoproteins. GPA is the carrier of blood group M and N specificities, while GPB accounts for S and U specificities. GPA and GPB provide the cells with a large mucin like surface and it has been suggested this provides a barrier to cell fusion, so minimizing aggregation between red blood cells in the circulation.

Alternate Names

CD235a, GPA, GYPA

Entrez Gene IDs

2993 (Human)

Gene Symbol

GYPA

UniProt

Additional Glycophorin A Products

Product Documents for Glycophorin A Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Glycophorin A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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