Glycophorin C Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-97086

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Glycophorin C Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human Glycophorin C (Accession#: NP_002092.1; Met1-Met57)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for Glycophorin C Antibody

Western Blot: Glycophorin C Antibody [NBP2-97086]

Western Blot: Glycophorin C Antibody [NBP2-97086]

Western Blot: Glycophorin C Antibody [NBP2-97086] - Anti-Glycophorin C rabbit polyclonal antibody at 1:500 dilution. Lane A: TF-1 Whole Cell Lysate. Lane B: K562 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa
Immunohistochemistry-Paraffin: Glycophorin C Antibody [NBP2-97086]

Immunohistochemistry-Paraffin: Glycophorin C Antibody [NBP2-97086]

Immunohistochemistry-Paraffin: Glycophorin C Antibody [NBP2-97086] - Immunochemical staining of human Glycophorin C in human placenta with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunoprecipitation: Glycophorin C Antibody [NBP2-97086]

Immunoprecipitation: Glycophorin C Antibody [NBP2-97086]

Immunoprecipitation: Glycophorin C Antibody [NBP2-97086] - Glycophorin C was immunoprecipitated using: Lane A: 0.5 mg TF-1 Whole Cell Lysate. Lane B: 0.5 mg K562 Whole Cell Lysate. 2 ul anti-Glycophorin C rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Glycophorin C rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 37 kDa

Applications for Glycophorin C Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:1000-1:10000

Immunoprecipitation

0.5-2uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Glycophorin C

Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, butplays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations havebeen described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duchantigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin Cprotein has very little homology with glycophorins A and B. (provided by RefSeq)

Alternate Names

CD236, CD236 antigen, CD236R, GE, GLPC, glycoconnectin, glycophorin C (Gerbich blood group), glycophorin-C, Glycophorin-D, Glycoprotein beta, GPCMGC126191, GPD, GYPD, MGC117309, MGC126192, PAS-2, PAS-2', Sialoglycoprotein D

Gene Symbol

GYPC

Additional Glycophorin C Products

Product Documents for Glycophorin C Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Glycophorin C Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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