Glyoxalase I Antibody (266)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-43618

Novus Biologicals, part of Bio-Techne
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NBP2-43618
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Bovine (90%), Mouse (92%), Rat (91%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 266

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Glyoxalase I Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Glyoxalase I. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (82%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

21 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Glyoxalase I Antibody (266)

Western Blot: Glyoxalase I Antibody (266) [NBP2-43618]

Western Blot: Glyoxalase I Antibody (266) [NBP2-43618]

Western Blot: Glyoxalase I Antibody (266) [NBP2-43618] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Glyoxalase I antibody [266] diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (266) [NBP2-43618]

Immunocytochemistry/ Immunofluorescence: Glyoxalase I Antibody (266) [NBP2-43618]

Immunocytochemistry/Immunofluorescence: Glyoxalase I Antibody (266) [NBP2-43618] - Glyoxalase I Antibody (266) detects Glyoxalase I protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Glyoxalase I stained by Glyoxalase I Antibody (266) diluted at 1:100. Blue: Hoechst 33342 staining. Scale bar= 10 um.
Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618]

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618]

Immunohistochemistry-Paraffin: Glyoxalase I Antibody (266) [NBP2-43618] - Glyoxalase I Antibody (266) detects Glyoxalase I protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human ovarian cancer.Glyoxalase I stained by Glyoxalase I Antibody (266) diluted at 1:200.
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Applications for Glyoxalase I Antibody (266)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Glyoxalase I

Lactoylglutathione lyase, also known as GLO1, is a member of the glyoxalase I family that is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. It is believed that inactivation of GLO1 in endothelial cells contributes to vascular complications in diabetes. GLO1 has also been shown to extend lifespan in C. elegans, and has been shown to be upregulated in malignant cells.

Alternate Names

Aldoketomutase, GLO1, GLOD1, Glx I, GLYI, Methylglyoxalase

Gene Symbol

GLO1

Additional Glyoxalase I Products

Product Documents for Glyoxalase I Antibody (266)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Glyoxalase I Antibody (266)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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