Glypican 3 Antibody (GPC3/863)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44486
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # GPC3/863
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant full-length human Glypican 3 protein (Uniprot: P51654)
Localization
Cytoplasmic
Specificity
Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
Marker
Hepatocellular Carcinoma Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
67 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47762)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Glypican 3 Antibody (GPC3/863)
Immunocytochemistry/ Immunofluorescence: Glypican 3 Antibody (GPC3/863) [NBP2-44486]
Immunocytochemistry/Immunofluorescence: Glypican 3 Antibody (GPC3/863) [NBP2-44486] - ICC/IF analysis of MeOH-fixed HepG2 cells labeling Glypican-3 with followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)Immunohistochemistry-Paraffin: Glypican 3 Antibody (GPC3/863) [NBP2-44486]
Immunohistochemistry-Paraffin: Glypican 3 Antibody (GPC3/863) [NBP2-44486] - Rat Lung stained with Glypican-3 Monoclonal Antibody (GPC3/863)Flow Cytometry: Glypican 3 Antibody (GPC3/863) [NBP2-44486]
Flow Cytometry: Glypican 3 Antibody (GPC3/863) [NBP2-44486] - Flow Cytometric Analysis of PFA-fixed HepG2 cells using Glypican 3 Antibody (GPC3/863) followed by Goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).Applications for Glypican 3 Antibody (GPC3/863)
Application
Recommended Usage
Flow Cytometry
1-2 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Glypican 3
Additional Glypican 3 Products
Product Documents for Glypican 3 Antibody (GPC3/863)
Product Specific Notices for Glypican 3 Antibody (GPC3/863)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...