Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI8G5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all GM-CSF Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 18-144 of human CSF2(NP_000749) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

14.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GM-CSF Antibody (OTI8G5) - Azide and BSA Free

Western Blot: GM-CSF Antibody (OTI8G5)Azide and BSA Free [NBP2-70462]

Western Blot: GM-CSF Antibody (OTI8G5)Azide and BSA Free [NBP2-70462]

Western Blot: GM-CSF Antibody (OTI8G5) - Azide and BSA Free [NBP2-70462] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GM-CSF.
Immunohistochemistry: GM-CSF Antibody (OTI8G5) - Azide and BSA Free [NBP2-70462]

Immunohistochemistry: GM-CSF Antibody (OTI8G5) - Azide and BSA Free [NBP2-70462]

Immunohistochemistry: GM-CSF Antibody (OTI8G5) - Azide and BSA Free [NBP2-70462] - Analysis of Human pancreas tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
Immunohistochemistry: GM-CSF Antibody (OTI8G5) - Azide and BSA Free [NBP2-70462]

Immunohistochemistry: GM-CSF Antibody (OTI8G5) - Azide and BSA Free [NBP2-70462]

Immunohistochemistry: GM-CSF Antibody (OTI8G5) - Azide and BSA Free [NBP2-70462] - Analysis of Carcinoma of Human liver tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)
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Applications for GM-CSF Antibody (OTI8G5) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GM-CSF

The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.

Long Name

Granulocyte Macrophage Growth Factor

Alternate Names

CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim

Gene Symbol

CSF2

Additional GM-CSF Products

Product Documents for GM-CSF Antibody (OTI8G5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GM-CSF Antibody (OTI8G5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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