GM-CSF Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB600-632
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse
Applications
Validated:
ELISA, Immunohistochemistry, Western Blot
Cited:
IF/IHC, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit Serum
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
This whole rabbit serum was prepared by repeated immunizations with full length recombinant human GM-CSF. (Uniprot: P04141)
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 31220744).
Localization
Secreted
Specificity
In ELISA and other immunoreactive assays, this antiserum will recognize both native and recombinant human GM-CSF in cell supernatants and certain body fluids.
Clonality
Polyclonal
Host
Rabbit
Isotype
Serum
Description
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Applications for GM-CSF Antibody
Application
Recommended Usage
ELISA
1:1000-1:5000
Immunohistochemistry
1:10-1:500
Western Blot
1:500-1:2000
Application Notes
This antiserum against Human GM-CSF has been tested for use in immunoblotting. Reactivity is also expected in neutralizations, radioimmunoassay, ELISA and immunohistochemistry. This antibody is useful for neutralization of human GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Antiserum
Preservative
No Preservative
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: GM-CSF
Long Name
Granulocyte Macrophage Growth Factor
Alternate Names
CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim
Entrez Gene IDs
1437 (Human)
Gene Symbol
CSF2
UniProt
Additional GM-CSF Products
Product Documents for GM-CSF Antibody
Product Specific Notices for GM-CSF Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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