GM-CSF Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-42743
Key Product Details
Species Reactivity
Mouse
Applications
ELISA, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
This purified GM-CSF Antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse GM-CSF protein. (Uniprot: P01587)
Specificity
In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse GM-CSF in cell supernatants and certain body fluids. This antibody is useful for neutralization of mouse GM-CSF in bioassays. For neutralization, it is recommended to incubate the sample with a 1:400 dilution of the antiserum for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Purified by a multi-step process which includes delipidation, salt fractionation, and heat treatment.
Purified by a multi-step process which includes delipidation, salt fractionation, and heat treatment.
Scientific Data Images for GM-CSF Antibody - Azide and BSA Free
![Western Blot: GM-CSF AntibodyAzide and BSA Free [NBP1-42743]](https://resources.bio-techne.com/images/products/GM-CSF-Antibody-Western-Blot-NBP1-42743-img0005.jpg "Western Blot: GM-CSF AntibodyAzide and BSA Free [NBP1-42743]")
Western Blot: GM-CSF AntibodyAzide and BSA Free [NBP1-42743]
Western Blot: GM-CSF Antibody [NBP1-42743] - Analysis of a band 14 kDa in size corresponding to recombinant mouse GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer.
GM-CSF Antibody - Azide and BSA Free
Western blot using anti-Mouse GM-CSF antibody shows detection of a band ~14 kDa in size corresponding to recombinant mouse GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight(TM)649 conjugated anti-Rabbit IgGApplications for GM-CSF Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:1000-1:5000
Western Blot
1:500-1:2000
Application Notes
This purified antibody has been tested in western blot and is suitable for use in neutralizations and ELISA. Reactivity is also expected in radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/ul by the LAL method. By western blot a band approximately 14.3 kDa in size corresponding to mouse GM-CSF protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: GM-CSF
Long Name
Granulocyte Macrophage Growth Factor
Alternate Names
CSF-2, CSF2, GMCSF, Molgramostim, Sargramostim
Gene Symbol
CSF2
UniProt
Additional GM-CSF Products
Product Documents for GM-CSF Antibody - Azide and BSA Free
Product Specific Notices for GM-CSF Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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