GM130/GOLGA2 Antibody (M342)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23353
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # M342
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone M342 was generated from a recombinant protein corresponding to amino acid residues in the C-terminal region of human GM130/GOLGA2. This sequence has high homology to similar regions in rat and mouse GM130/GOLGA2. [UniProt# Q08379]
Specificity
This antibody detects a 130 kDa protein corresponding to the apparent molecular mass of GM130/GOLGA2 on SDS-PAGE immunoblots of mouse C2C12 cells. In immunocytochemistry, anti-GM130 specifically stains Golgi in mouse C2C12 cells.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Stable for at least 1 year at -20°C.
Scientific Data Images for GM130/GOLGA2 Antibody (M342)
Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody (M342) [NBP3-23353] -
Immunocytochemistry/Immunofluorescence: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Immunocytochemical labeling of GM130/GOLGA2 in C2C12 cells. The cells were double-labeled with GM130/GOLGA2 (golgi protein) mouse monoclonal and nSMase-3 rabbit polyclonal antibodies, then detected using appropriate secondary antibody conjugated to Cy2 or Cy3.
Western Blot: GM130/GOLGA2 Antibody (M342) [NBP3-23353] -
Western Blot: GM130/GOLGA2 Antibody (M342) [NBP3-23353] - Western blot analysis of GM130/GOLGA2 expression in human cells: MCF7 breast carcinoma (lane 1), A549 adenocarcinoma (lane 2), and MDA-MB-231 breast carcinoma (lane 3). The blot was probed with mouse monoclonal anti-GM130/GOLGA2 (C-terminal region) at 1:500.Applications for GM130/GOLGA2 Antibody (M342)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50
Western Blot
1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS, 1 mg/ml BSA and 50% glycerol
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: GM130/GOLGA2
Long Name
Golgin A2
Alternate Names
GOLGA2, Golgin-95, SY11 Protein
Gene Symbol
GOLGA2
Additional GM130/GOLGA2 Products
Product Documents for GM130/GOLGA2 Antibody (M342)
Product Specific Notices for GM130/GOLGA2 Antibody (M342)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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