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Key Product Details

Species Reactivity

Validated:

Rat

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # NN 2C10/1

Format

BSA Free

Concentration

1 mg/ml

Product Summary for GM130/GOLGA2 Antibody (NN 2C10/1) - BSA Free

Immunogen

Recombinant purified rat GM130/GOLGA2

Localization

Peripheral membrane protein associated with Golgi stacks

Specificity

Does not cross react with human protein.

Marker

Golgi Apparatus Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Applications for GM130/GOLGA2 Antibody (NN 2C10/1) - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:10 - 1:500

Immunoprecipitation

1:10 - 1:500

Western Blot

1:100 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GM130/GOLGA2

The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. GM130 encodes a member of the Golgin protein family and is localised to the Golgi. The protein encoded by GM130 has been postulated to play roles in the stacking of Golgi cisternae, maintaining cis-Golgi structure and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. GM130 is part of a larger oligomeric complex which interacts with p115, GORASP1/GRASP65 and RAB1B that has been activated by GTP-binding. GM130 is useful as a cis-Golgi marker in immunohistochemistry.

Long Name

Golgin A2

Alternate Names

GOLGA2, Golgin-95, SY11 Protein

Entrez Gene IDs

2801 (Human); 64528 (Rat)

Gene Symbol

GOLGA2

Additional GM130/GOLGA2 Products

Product Documents for GM130/GOLGA2 Antibody (NN 2C10/1) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GM130/GOLGA2 Antibody (NN 2C10/1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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