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GM130/GOLGA2 Antibody (SR1125)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21627

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-21627-100ul
NBP3-21627-50ul

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR1125

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthesized peptide derived from human GM130/GOLGA2 (Uniprot #: Q08379)

Specificity

Detects endogenous levels of total GM130/GOLGA2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

130 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GM130/GOLGA2 Antibody (SR1125)

Immunohistochemistry: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] -

Immunohistochemistry: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] - Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using NBP3-21627.

Western Blot: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] -

Western Blot: GM130/GOLGA2 Antibody (SR1125) [NBP3-21627] - Western blot analysis using NBP3-21627 in (1) HeLa cell lysate; (2) MCF-7 cell lysate.

Applications for GM130/GOLGA2 Antibody (SR1125)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:50

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GM130/GOLGA2

Golgi Matrix Protein (GM130) is peripheral cytoplamic protein that is bound to the Golgi membranes. It has been implicated as a maintainer of cis-Golgi structure (1). During mitosis, it regulates the disassembly and reassembly of the Golgi complex. It is also linked to docking and fusion of coatomer (COP I) coated vesicles to the Golgi membrane (2). The COOH-terminal domain of GM130 is highly homologous to Golgi human auto-antigen, golgin-95. GM130 interacts specifically and GTP-dependent manner with Rab1b protein, a regulator of anterograde traffic between ER and Golgi membranes (3). GM130 is also activator of YSK1, an essential player in cell migration.

Long Name

Golgin A2

Alternate Names

GOLGA2, Golgin-95, SY11 Protein

Gene Symbol

GOLGA2

Additional GM130/GOLGA2 Products

Product Documents for GM130/GOLGA2 Antibody (SR1125)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GM130/GOLGA2 Antibody (SR1125)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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