GOLGA3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91952

Novus Biologicals, part of Bio-Techne
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NBP1-91952
NBP1-91952-25ul
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GOLGA3 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: STRGTYGILSKTVGTQDTPYMVNGQEIPADTLGQFPSIKDVLQAAAAEHQDQGQEVNGEVRSRRDSICSSVSLESSAAETQEEMLQVLKEKMRLEG

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GOLGA3 Antibody

Western Blot: GOLGA3 Antibody [NBP1-91952]

Western Blot: GOLGA3 Antibody [NBP1-91952]

Western Blot: GOLGA3 Antibody [NBP1-91952] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: GOLGA3 Antibody [NBP1-91952]

Immunocytochemistry/ Immunofluorescence: GOLGA3 Antibody [NBP1-91952]

Immunocytochemistry/Immunofluorescence: GOLGA3 Antibody [NBP1-91952] - Staining of human cell line U-2 OS shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952]

Immunohistochemistry-Paraffin: GOLGA3 Antibody [NBP1-91952] - Staining of human tonsil shows moderate to strong cytoplasmic positivity in germinal center cells.
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Applications for GOLGA3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GOLGA3

GOLGA3 is a member of the golgin family of proteins which are localized to the Golgi. It has been postulated to play a role in nuclear transport and Golgi apparatus localization.

Alternate Names

GCP170Golgi peripheral membrane protein, golgi autoantigen, golgin subfamily a, 3, Golgi complex-associated protein of 170 kD, Golgi complex-associated protein of 170 kDa, Golgi membrane associated protein, golgin A3, Golgin subfamily A member 3, golgin-160, golgin-165, male enhanced antigen-2, MEA-2, SY2/SY10 protein

Gene Symbol

GOLGA3

Additional GOLGA3 Products

Product Documents for GOLGA3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GOLGA3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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