GOLM1 Antibody (9C2)

Western Blot: GOLM1 Antibody (9C2) [NBP3-26550] -

Western Blot: GOLM1 Antibody (9C2) [NBP3-26550] - Positive Western Blot detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate.
All lanes: GOLM1 Antibody at 1.3ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 46, 45 KDa
Observed band size: 80 KDa

Flow Cytometry: GOLM1 Antibody (9C2) [NBP3-26550] -

Flow Cytometry: GOLM1 Antibody (9C2) [NBP3-26550] - Overlay histogram showing Hela cells stained with GOLM1 Antibody (9C2) (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.