GOLM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88774

Novus Biologicals, part of Bio-Techne
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NBP1-88774
NBP1-88774-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GOLM1 Antibodies »

Product Summary for GOLM1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCE

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%), Rat (86%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GOLM1 Antibody

Western Blot: GOLM1 Antibody [NBP1-88774]

Western Blot: GOLM1 Antibody [NBP1-88774]

Western Blot: GOLM1 Antibody [NBP1-88774] - Analysis in control (vector only transfected HEK293T lysate) and GOLM1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-88774]

Immunocytochemistry/ Immunofluorescence: GOLM1 Antibody [NBP1-88774]

Immunocytochemistry/Immunofluorescence: GOLM1 Antibody [NBP1-88774] - Staining of human cell line U-251 MG shows localization to the Golgi apparatus.
Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88774]

Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88774]

Immunohistochemistry-Paraffin: GOLM1 Antibody [NBP1-88774] - Staining in human stomach and skeletal muscle tissues using anti-GOLM1 antibody. Corresponding GOLM1 RNA-seq data are presented for the same tissues.
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Applications for GOLM1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GOLM1

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by GOLM1 is a type II Golgi transmembrane protein. It processes protein synthesized in the rough endoplasmic reticulum and assists in the transport of protein cargo through the Golgi apparatus. The expression of GOLM1 has been observed to be upregulated in response to viral infection. Two alternatively spliced transcript variants encoding the same protein have been described for GOLM1.

Alternate Names

bA379P1.3, FLJ23608, golgi membrane protein 1, Golgi membrane protein GP73, Golgi phosphoprotein 2C9orf155, golgi protein, 73-kD, GOLPH2 FLJ22634, GP73, GP73 chromosome 9 open reading frame 155, PSEC0257

Gene Symbol

GOLM1

Additional GOLM1 Products

Product Documents for GOLM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GOLM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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