GOT2 Antibody (8B11) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-59428

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Kappa Clone # 8B11

Format

BSA Free

Concentration

1 mg/ml

View all GOT2 Antibodies »

Product Specifications

Immunogen

Recombinant human GOT2 (30-430aa) purified from E.coli.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 30067865).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3 Kappa

Scientific Data Images for GOT2 Antibody (8B11) - BSA Free

Western Blot: GOT2 Antibody (8B11)BSA Free [NBP2-59428]

Western Blot: GOT2 Antibody (8B11)BSA Free [NBP2-59428]

Western Blot: GOT2 Antibody (8B11) [NBP2-59428] - The recombinant proteins (20ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GOT2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human GOT1 Lane 2.: Recombinant Human GOT2
Immunocytochemistry/ Immunofluorescence: GOT2 Antibody (8B11) - BSA Free [NBP2-59428]

Immunocytochemistry/ Immunofluorescence: GOT2 Antibody (8B11) - BSA Free [NBP2-59428]

Immunocytochemistry/Immunofluorescence: GOT2 Antibody (8B11) [NBP2-59428] - Analysis of GOT2 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human GOT2 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).
Flow Cytometry: GOT2 Antibody (8B11) - BSA Free [NBP2-59428]

Flow Cytometry: GOT2 Antibody (8B11) - BSA Free [NBP2-59428]

Flow Cytometry: GOT2 Antibody (8B11) [NBP2-59428] - Analysis of GOT2 in HepG2 cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).
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Applications for GOT2 Antibody (8B11) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1037

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GOT2

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Alternate Names

aspartate aminotransferase, mitochondrial, EC 2.6.1, EC 2.6.1.1, FABP-1, FABPpm, Fatty acid-binding protein, FLJ40994, Glutamate oxaloacetate transaminase 2, glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2), KAT4, KATIV, kynurenine aminotransferase IV, mAspAT, mitAAT, Plasma membrane-associated fatty acid-binding protein, Transaminase A

Gene Symbol

GOT2

Additional GOT2 Products

Product Documents for GOT2 Antibody (8B11) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GOT2 Antibody (8B11) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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