Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (100%), Chicken (100%), Equine (100%), Orangutan (100%), Porcine (100%), Rabbit (100%), Xenopus (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GOT2 Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 380 to 430 of human Glutamic-Oxaloacetic Transaminase 2 using the numbering given in entry NP_002071.2 (GeneID 2806).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GOT2 Antibody

Western Blot: GOT2 Antibody [NBP2-32241]

Western Blot: GOT2 Antibody [NBP2-32241]

Western Blot: GOT2 Antibody [NBP2-32241] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-GOT2 antibody NBP2-32241 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241] - Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- GOT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241]

Immunohistochemistry-Paraffin: GOT2 Antibody [NBP2-32241] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- GOT2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Applications for GOT2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunoprecipitation Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GOT2

Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Alternate Names

aspartate aminotransferase, mitochondrial, EC 2.6.1, EC 2.6.1.1, FABP-1, FABPpm, Fatty acid-binding protein, FLJ40994, Glutamate oxaloacetate transaminase 2, glutamic-oxaloacetic transaminase 2, mitochondrial (aspartate aminotransferase2), KAT4, KATIV, kynurenine aminotransferase IV, mAspAT, mitAAT, Plasma membrane-associated fatty acid-binding protein, Transaminase A

Gene Symbol

GOT2

UniProt

NP_002071.2 (Human)

Additional GOT2 Products

Product Documents for GOT2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GOT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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