GPIHBP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB110-55293

Novus Biologicals, part of Bio-Techne
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NB110-55293
NB110-55293SS
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1 mg/ml

View all GPIHBP1 Antibodies »

Product Summary for GPIHBP1 Antibody

Immunogen

A synthetic peptide corresponding to an internal region [within residues 100-180] of human GPIHBP1. [Swiss-Prot# Q8IV16]

Localization

Cytoplasmic and Nuclear

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GPIHBP1 Antibody

Western Blot: GPIHBP1 Antibody [NB110-55293]

Western Blot: GPIHBP1 Antibody [NB110-55293]

Western Blot: GPIHBP1 Antibody [NB110-55293] - Detection of HDLBP in CHO transfected lysate using NB110-55293. Lane 1: empty vector Lane 2: HDLBP transfected lysate

Applications for GPIHBP1 Antibody

Application
Recommended Usage

Western Blot

2 ug/ml
Application Notes
This GPIHBP1 antibody is useful for Western blot analysis where a band is seen at ~19 kDa using CHO pgsA745 transfected cell lysates.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Glycine and 0.15M NaCl

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GPIHBP1

GPIHBP1 (glycosylphosphatidylinositol-anchored HDL-binding protein 1) is a glycosylphosphatidylinositol-anchored glycoprotein of capillary endothelial cells that shuttle lipoprotein lipase (LPL) from the interstitial spaces to capillary lumen, and is essential for triglyceride-rich lipoproteins metabolism in mammalian plasma. GPIHBP1 is localized on luminal/abluminal capillary endothelial cell surfaces where it is bound by a glycosylphosphatidylinositol anchor and associates strongly with LPL. It serves as LPL transporter from sub-endothelial spaces to luminal face of capillaries, enabling lipolysis of circulating triglycerides localized within plasma chylomicrons. It has high affinity for HDL and binds to LPL, chylomicrons as well as APOA5. In the absence of GPIHBP1, the stores of catalytically active LPL within tissues are normal, but the LPL is mislocalized to interstitial spaces and is absent from capillary lumen. LPL mislocalization interferes with lipoprotein lipolysis and causes chylomicronemia. In humans, loss of function GPIHBP1 mutations leads to familial chylomicronemia. It binds LPL and apoA-V strongly for serving as a platform for lipolysis within capillaries, particularly in tissues which show high expression levels for both GPIHBP1 and LPL genes, such as heart, skeletal muscle and adipose tissue. Gpihbp1-/Gpihbp1- knock out mice have shown that GPIHBP1-deficiency causes severe hypertriglyceridemia.

Long Name

Glycosylphosphatidylinositol Anchored High Density Lipoprotein Binding Protein 1

Alternate Names

GPI-Anchored HDL-Binding Protein 1, GPI-HBP1, HBP1, HYPL1D

Gene Symbol

GPIHBP1

Additional GPIHBP1 Products

Product Documents for GPIHBP1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GPIHBP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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