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GPR64 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84906

Novus Biologicals, part of Bio-Techne
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NBP1-84906
NBP1-84906-25ul

Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: SPTVSAPANVNTTSAPPVQTDIVNTSSISDLENQVLQMEKALSLGSLEPNLAGEMINQVSRLLHSPPDMLAPLAQRLLKVVDDIGLQLNFSNTTISLTSPSLALAVIRVNASSFNTTTFVAQDPANLQVSLETQAPE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GPR64 Antibody

Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906]

Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906]

Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906] - Staining in human epididymis and endometrium tissues using NBP1-84906 antibody. Corresponding ADGRG2 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906]

Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906]

Immunohistochemistry-Paraffin: GPR64 Antibody [NBP1-84906] - Staining of human endometrium, epididymis, liver and skeletal muscle using Anti-ADGRG2 antibody NBP1-84906 (A) shows similar protein distribution across tissues to independent antibody NBP2-55504 (B).
Western Blot: GPR64 Antibody [NBP1-84906]

Western Blot: GPR64 Antibody [NBP1-84906]

Western Blot: GPR64 Antibody [NBP1-84906] - Expression of selected genes in BCC subsets. Western blots for GPR64, TMEM98, FAT4, RAP1A and FN1 using whole cell extracts expression from Oct4hi, Octmed and Oct4low, isolated as for 'A'. The results represent four different independent experiments. Image collected and cropped by CiteAb from the following publication (https://www.nature.com/articles/s41598-017-18834-5), licensed under a CC-BY license.

Applications for GPR64 Antibody

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Use in WB reported in scientific literature (PMID: 29321622).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GPR64

GPR64 is an Orphan-B GPCR with an unknown ligand. HE6 is endogenously cleaved into two pieces at the GPCR proteolytic site (GPS) located adjacent to the first transmembrane helix as a mechanism to compartmentalize the cell adhesion and GPCR activation functions. GPR64 has been reported in the epididymis. ESTs have been isolated from embryo, kidney, placenta, skeletal muscle, and testis libraries.

Long Name

G Protein-coupled Receptor 64

Alternate Names

EDDM6, HE6, TM7LN2

Gene Symbol

ADGRG2

Additional GPR64 Products

Product Documents for GPR64 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GPR64 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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