GPR81 Antibody - BSA Free

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Analysis of anti-FKSG80 / GPR81 antibody with human colon, carcinoma.

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Skin, Melanoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human Ovary, Carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Human pancreas, carcinoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Paraffin: GPR81 Antibody - BSA Free [NLS2095]

Immunohistochemistry-Paraffin: GPR81 Antibody [NLS2095] - Breast, Carcinoma

Western Blot: GPR81 Antibody - BSA Free [NLS2095] -

Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Stable knockdown of GPR81 in MDA-MB-231 cells. Using a lentiviral system, GPR81 was stably knocked down in MDA-MB-231 cells with GPR81-specific short hairpin RNA (shGPR81 #1) & compared with cells containing scrambled shRNA as a control (shNT). Knockdown of GPR81 was confirmed by (a) western blotting & (b) RT-qPCR. The GPR81, MCT1, & MCT4 mRNA levels in the shGPR81 #1 cells are presented as the fold increase compared with those in shNT cells. Data are presented as the means ± s.d. (n = 3/group). **p < 0.01; Student’s t-test. (c) Lactate production in shNT & shGPR81 #1 cells. The cells were cultured for 24 h & lactate concentrations in the supernatants were determined (n = 3/group). **p < 0.01 (vs. shNT cells); Student’s t-test. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GPR81 Antibody - BSA Free [NLS2095] -

Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: GPR81 Antibody - BSA Free [NLS2095] -

Western Blot: GPR81 Antibody - BSA Free [NLS2095] - Expression of GPR81 in breast cancer cells. (a) Western blot analysis of GPR81 in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). Cell lysates were analyzed by immunoblotting using anti-GPR81, anti-MCT4, anti-MCT1, & anti-beta -actin antibodies. (b) Immunocytochemical analysis of GPR81 expression in breast cancer cells (MCF-7 & MDA-MB-231) & non-tumorigenic epithelial cells (MCF-10A). MCF-10A, MCF-7 & MDA-MB-231 cells were incubated with polyclonal antibodies against GPR81 followed by Alexa Fluor Plus 488-conjugated secondary antibody & visualized under a fluorescence microscope. Na/K ATPase was used as a cell membrane marker & the nuclei were stained with DAPI. Scale bar 10 μm. (c) MDA-MB-231 cells were cultured in the presence & absence of 20 mM lactate for 48 h, & cell lysates were analyzed by immunoblotting using anti-GPR81 & anti-beta -actin antibodies. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35428832), licensed under a CC-BY license. Not internally tested by Novus Biologicals.