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GPT2 Antibody (7A11)

Novus Biologicals, part of Bio-Techne | Catalog # H00084706-M04

Novus Biologicals, part of Bio-Techne
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H00084706-M04

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 7A11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

GPT2 (NP_597700, 358 a.a. ~ 456 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. NLHPEIKGQLVKLLSVRLCPPVSGQAAMDIVVNPPVAGEESFEQFSREKESVLGNLAKKAKLTEDLFNQVPGIHCNPLQGAMYAFPRIFIPAKAVEAAQ

Specificity

This product is specific for Human GPT2 monoclonal antibody (M04), clone 7A11 [Gene ID: 84706].

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for GPT2 Antibody (7A11)

Western Blot: GPT2 Antibody (7A11) [H00084706-M04]

Western Blot: GPT2 Antibody (7A11) [H00084706-M04]

Western Blot: GPT2 Antibody (7A11) [H00084706-M04] - Analysis of GPT2 expression in transfected 293T cell line by GPT2 monoclonal antibody (M04), clone 7A11. Lane 1: GPT2 transfected lysate (Predicted MW: 57.9 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: GPT2 Antibody (7A11) [H00084706-M04]

Immunocytochemistry/ Immunofluorescence: GPT2 Antibody (7A11) [H00084706-M04]

Immunocytochemistry/Immunofluorescence: GPT2 Antibody (7A11) [H00084706-M04] - Analysis of monoclonal antibody to GPT2 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: GPT2 Antibody (7A11) [H00084706-M04] - Detection limit for recombinant GST tagged GPT2 is 1 ng/ml as a capture antibody.

Applications for GPT2 Antibody (7A11)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Mouse monoclonal antibody raised against a partial recombinant GPT2.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GPT2

GPT (MIM 138200) and GPT2 (EC 2.6.1.2), also known as alanine transaminases, are pyridoxal enzymes that catalyze the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. By mediating the conversion of these 4 major intermediate metabolites, these transaminases have roles in gluconeogenesis and in amino acid metabolism.[supplied by OMIM]

Alternate Names

AAT2, alanine aminotransferase 2, ALT2EC 2.6.1.2, Glutamate pyruvate transaminase 2, glutamic pyruvate transaminase (alanine aminotransferase) 2, Glutamic--alanine transaminase 2, glutamic-pyruvate transaminase 2, Glutamic--pyruvic transaminase 2, GPT 2

Gene Symbol

GPT2

Additional GPT2 Products

Product Documents for GPT2 Antibody (7A11)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GPT2 Antibody (7A11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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