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Gr-1/Ly-6G Antibody (3D6E10)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-27164

Novus Biologicals, part of Bio-Techne
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NBP3-27164-0.025ml
NBP3-27164-0.1ml

Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3D6E10

Concentration

1 mg/ml

Product Specifications

Immunogen

Purified recombinant fragment of human Gr-1/Ly-6G (AA: 20-104) expressed in E. Coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

13.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Gr-1/Ly-6G Antibody (3D6E10)

Flow Cytometry: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] -

Flow Cytometry: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis of Jurkat cells using Gr-1/Ly-6G mouse mAb (green) and negative control (red).

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] -

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis using Gr-1/Ly-6G mouse mAb against Jurkat (1), MCF-7 (2), A549 (3),K562 (4),Hela (5),C6 (6),COS-7 (7),and NIH/3T3 (8) cell lysate.

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] -

Western Blot: Gr-1/Ly-6G Antibody (3D6E10) [NBP3-27164] - Analysis using Gr-1/Ly-6G mAb against HEK293-6e (1) and Gr-1/Ly-6G (AA: 20-104)-hIgGFc transfected HEK293-6e (2) cell lysate.

Applications for Gr-1/Ly-6G Antibody (3D6E10)

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ly-6G/Ly-6C (Gr-1)

The mouse Gr-1 antigen is a GPI-linked myeloid differentiation marker also known as Ly-6G. Its cellular expression is restricted to monocytes within bone and on neutrophils in peripheral organs.

Long Name

A Myeloid Differentiation Antigen

Alternate Names

Ly-6C, Ly-6G, Ly6G

Gene Symbol

LY6G6D

Additional Ly-6G/Ly-6C (Gr-1) Products

Product Documents for Gr-1/Ly-6G Antibody (3D6E10)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Gr-1/Ly-6G Antibody (3D6E10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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