GR/NR3C1 Antibody (BuGR2) [PE]
Novus Biologicals, part of Bio-Techne | Catalog # NB300-731PE
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Guinea Pig, Rabbit, Sheep, Yeast
Cited:
Human
Applications
Validated:
Block/Neutralize, Chromatin Immunoprecipitation (ChIP), Flow Cytometry, Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence
Label
PE (Excitation = 488 nm, Emission = 575 nm)
Antibody Source
Monoclonal Mouse IgG2A Clone # BuGR2
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Partially purified rat GR.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Localization
Cytoplasmic in the absence of ligand; nuclear after ligand-binding.
Specificity
Using enzymatic digestion analysis, has been shown to react with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for GR/NR3C1 Antibody (BuGR2) [PE]
Flow Cytometry: GR/NR3C1 Antibody (BuGR2) [PE] [NB300-731PE] - An intracellular stain was performed on Jurkat cells with GR/NR3C1 (BuGR2) antibody NB300-731PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Phycoerthrin.
Applications for GR/NR3C1 Antibody (BuGR2) [PE]
Application
Recommended Usage
Block/Neutralize
Optimal dilutions of this antibody should be experimentally determined.
Chromatin Immunoprecipitation (ChIP)
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Gel Super Shift Assays
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry-Paraffin
Optimal dilutions of this antibody should be experimentally determined.
Immunoprecipitation
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: GR/NR3C1
Long Name
Glucocorticoid Receptor
Alternate Names
NR3C1
Gene Symbol
NR3C1
Additional GR/NR3C1 Products
Product Documents for GR/NR3C1 Antibody (BuGR2) [PE]
Product Specific Notices for GR/NR3C1 Antibody (BuGR2) [PE]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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