GR/NR3C1 Antibody (GT1073)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13630

Novus Biologicals, part of Bio-Techne
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NBP3-13630
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (90%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # GT1073

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GR/NR3C1 Antibodies »

Product Summary for GR/NR3C1 Antibody (GT1073)

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human GR/NR3C1. The exact sequence is proprietary.

Reactivity Notes

Dog (88%), Cat (89%), Pig (83%), Sheep (89%), Guinea pig (85%)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

86 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Centrifuge briefly prior to opening.

Scientific Data Images for GR/NR3C1 Antibody (GT1073)

Western Blot: GR/NR3C1 Antibody (GT1073) [NBP3-13630]

Western Blot: GR/NR3C1 Antibody (GT1073) [NBP3-13630]

Western Blot: GR/NR3C1 Antibody (GT1073) [NBP3-13630] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GR/NR3C1 antibody [GT1073] (NBP3-13630) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.
Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT1073) [NBP3-13630]

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT1073) [NBP3-13630]

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT1073) [NBP3-13630] - Rat brain. GR/NR3C1 stained by GR/NR3C1 antibody [GT1073] (NBP3-13630) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT1073) [NBP3-13630]

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT1073) [NBP3-13630]

Immunohistochemistry-Paraffin: GR/NR3C1 Antibody (GT1073) [NBP3-13630] - Mouse intestine. GR/NR3C1 stained by GR/NR3C1 antibody [GT1073] (NBP3-13630) diluted at 1:1000. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Applications for GR/NR3C1 Antibody (GT1073)

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GR/NR3C1

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Long Name

Glucocorticoid Receptor

Alternate Names

NR3C1

Gene Symbol

NR3C1

Additional GR/NR3C1 Products

Product Documents for GR/NR3C1 Antibody (GT1073)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GR/NR3C1 Antibody (GT1073)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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