GR/NR3C1 Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB120-3579
Key Product Details
Species Reactivity
Human, Mouse, Rat, Primate
Applications
Chromatin Immunoprecipitation (ChIP), Gel Super Shift Assays, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR.
Localization
Cytoplasmic in the absence of ligand; nuclear after ligand-binding.
Specificity
NB120-3579 detects glucocorticoid receptor (GR) from human, mouse and rat tissues.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GR/NR3C1 Antibody - Azide and BSA Free
![Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody - Azide and BSA Free [NB120-3579]](https://resources.bio-techne.com/images/products/GR-NR3C1-Antibody-59-Immunocytochemistry-Immunofluorescence-NB120-3579-img0002.jpg "Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody - Azide and BSA Free [NB120-3579]")
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody - Azide and BSA Free [NB120-3579]
Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in Hela Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:100 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.![Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody - Azide and BSA Free [NB120-3579]](https://resources.bio-techne.com/images/products/GR-NR3C1-Antibody-59-Immunocytochemistry-Immunofluorescence-NB120-3579-img0001.jpg "Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody - Azide and BSA Free [NB120-3579]")
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody - Azide and BSA Free [NB120-3579]
Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody (59) [NB120-3579] - Analysis of Glucocorticoid Receptor using Glucocorticoid Receptor Polyclonal Antibody (59) shows staining in A2058 Cells. Glucocorticoid Receptor (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Glucocorticoid Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.Applications for GR/NR3C1 Antibody - Azide and BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Gel Super Shift Assays
1:1 - 1:100
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1 ug/ml
Immunohistochemistry-Frozen
1 ug/ml
Immunoprecipitation
5 ug/ml
Western Blot
5 ug/ml
Application Notes
WB: Detects an approx. 97 kDa protein representing GR as well as two other unidentified proteins at approx. 135 - 145 kDa from rat liver extract. IHC: Staining of GR in rat hippocampus using NB120-3579 results in staining primarily in the nucleus. NB120-3579 does not disrupt receptor-DNA interactions.
See Simple Western Antibody Database for Simple Western validation: tested in primate corpus luteum; antibody dilution of 1:10; separated by size; multiple isoform bands detected
See Simple Western Antibody Database for Simple Western validation: tested in primate corpus luteum; antibody dilution of 1:10; separated by size; multiple isoform bands detected
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
Reconstitute with PBS.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GR/NR3C1
Long Name
Glucocorticoid Receptor
Alternate Names
NR3C1
Gene Symbol
NR3C1
UniProt
Additional GR/NR3C1 Products
Product Documents for GR/NR3C1 Antibody - Azide and BSA Free
Product Specific Notices for GR/NR3C1 Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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