GR/NR3C1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89733

Novus Biologicals, part of Bio-Techne
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NBP1-89733
NBP1-89733-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GR/NR3C1 Antibodies »

Product Summary for GR/NR3C1 Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: DSKESLTPGREENPSSVLAQERGDVMDFYKTLRGGATVKVSASSPSLAVASQSDSKQRRLLVDFPKGSVSNAQQPDLSKAVSLSMGLYMGETETKVMGNDLGFPQQGQISLSSGETDLKLLEESIANLNRSTSVPENPKSSASTAVSA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GR/NR3C1 Antibody

Western Blot: GR/NR3C1 Antibody [NBP1-89733]

Western Blot: GR/NR3C1 Antibody [NBP1-89733]

Western Blot: GR/NR3C1 Antibody [NBP1-89733] - Analysis in control (vector only transfected HEK293T lysate) and NR3C1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody [NBP1-89733]

Immunocytochemistry/ Immunofluorescence: GR/NR3C1 Antibody [NBP1-89733]

Immunocytochemistry/Immunofluorescence: GR/NR3C1 Antibody [NBP1-89733] - Staining of human cell line A-431 shows positivity in nucleus but not nucleoli and cytoplasm. Antibody staining is shown in green.
Western Blot: GR/NR3C1 Antibody [NBP1-89733]

Western Blot: GR/NR3C1 Antibody [NBP1-89733]

Western Blot: GR/NR3C1 Antibody [NBP1-89733] - Analysis in human cell lines A-549 and Caco-2 using anti-NR3C1 antibody. Corresponding NR3C1 RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.

Applications for GR/NR3C1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GR/NR3C1

Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor. The corresponding gene for the glucocorticoid receptor is NR3C1.

Long Name

Glucocorticoid Receptor

Alternate Names

NR3C1

Gene Symbol

NR3C1

Additional GR/NR3C1 Products

Product Documents for GR/NR3C1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GR/NR3C1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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