Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all GRAMD1A Antibodies »

Product Specifications

Immunogen

The immunogen this antibody was made to, maps to a region between residue 674 to 724 of human GRAM Domain Containing 1A using the numbering given in entry NP_065946.2 (GeneID 57655).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRAMD1A Antibody

Western Blot: GRAMD1A Antibody [NBP2-32148]

Western Blot: GRAMD1A Antibody [NBP2-32148]

Western Blot: GRAMD1A Antibody [NBP2-32148] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, MCF7, Jurkat, and K562 cells. Antibodies: Affinity purified rabbit anti-GRAMD1A antibody NBP2-32148 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: GRAMD1A Antibody [NBP2-32148]

Immunoprecipitation: GRAMD1A Antibody [NBP2-32148]

Immunoprecipitation: GRAMD1A Antibody [NBP2-32148] - Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-GRAMD1A antibody NBP2-32148 used for IP at 6 ug/mg lysate. For blotting immunoprecipitated GRAMD1A, NBP2-32148 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for GRAMD1A Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:1000 - 1:5000
Application Notes
Western blot of lysates performed using standard western blot reagents and 4-8% SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GRAMD1A

Alternate Names

FLJ22411, FLJ90346, GRAM domain containing 1A, GRAM domain-containing protein 1A, KIAA1533

Gene Symbol

GRAMD1A

UniProt

NP_065946.2 (Human)

Additional GRAMD1A Products

Product Documents for GRAMD1A Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GRAMD1A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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