Granulin Antibody (OTI3H6) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70847

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3H6

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Granulin Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 327-593 of human GRN(NP_002078) produced in E.coli.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Granulin Antibody (OTI3H6) - Azide and BSA Free

Western Blot: Granulin Antibody (OTI3H6)Azide and BSA Free [NBP2-70847]

Western Blot: Granulin Antibody (OTI3H6)Azide and BSA Free [NBP2-70847]

Western Blot: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRN (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRN.
Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847]

Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847]

Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Human colon tissue using anti-GRN mouse monoclonal antibody.
Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847]

Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847]

Immunohistochemistry: Granulin Antibody (OTI3H6) - Azide and BSA Free [NBP2-70847] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GRN mouse monoclonal antibody.
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Applications for Granulin Antibody (OTI3H6) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Granulin

Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc. Epithelins 1 and 2 are synonymous with granulins A and B, respectively. Both the peptides and intact granulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis.

Alternate Names

acrogranin, GEP, GP88, granulin, granulin-epithelin, granulins, PC cell-derived growth factor, PCDGF, PEPI, PGRN, Proepithelin, progranulin

Gene Symbol

GRN

Additional Granulin Products

Product Documents for Granulin Antibody (OTI3H6) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Granulin Antibody (OTI3H6) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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