Granzyme B Antibody (B18.1) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97525

Novus Biologicals, part of Bio-Techne
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NBP1-97525
NBP1-97525-0.025mg
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Flow Cytometry, IF/IHC, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # B18.1

Format

BSA Free

Concentration

1.0 mg/ml

View all Granzyme B Antibodies »

Product Specifications

Immunogen

Recombinant human granzyme B (aa 7-227).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Granzyme B Antibody (B18.1) - BSA Free

Flow Cytometry: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]

Flow Cytometry: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]

Flow Cytometry: Granzyme B Antibody (B18.1) [NBP1-97525] - An intracellular stain was performed on U937 cells with Granzyme B [B18.1] Antibody NBP1-97525AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Western Blot: Granzyme B Antibody (B18.1)BSA Free [NBP1-97525]

Western Blot: Granzyme B Antibody (B18.1)BSA Free [NBP1-97525]

Western Blot: Granzyme B Antibody (B18.1) [NBP1-97525] - Detection of recombinant granzyme B. Granzyme B migrates as a 27 kDa species. Method: Recombinant granzyme B was resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and probed with the monoclonal anti-granzyme B antibody B18.1 at 1ug/ml. Proteins were visualized using a peroxidase-conjugated antibody to mouse IgG (SBA) and a chemiluminescence detection system
Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]

Immunocytochemistry/ Immunofluorescence: Granzyme B Antibody (B18.1) - BSA Free [NBP1-97525]

Immunocytochemistry/Immunofluorescence: Granzyme B Antibody (B18.1) [NBP1-97525] - Human T cells were plated onto polylysine treated glass slides, fixed and permeabilized in methanol at -20oC for 5 min, then in acetone at -20oC for 30 sec. After 3 washes in PBS, 0.1% BSA, slides were incubated for 1 hr at room temperature (RT) with 20 ug/ml of B18.4 antibody in PBS, 0.1% BSA. After rinsing in PBS, a FITC-conjugated anti-mouse IgG was added for 30 min., slides washed again in PBS and visualized using a fluorescence microscope.
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Applications for Granzyme B Antibody (B18.1) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Granzyme B

Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.

Alternate Names

CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT

Gene Symbol

GZMB

Additional Granzyme B Products

Product Documents for Granzyme B Antibody (B18.1) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Granzyme B Antibody (B18.1) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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