Granzyme B Antibody (GB11)
Novus Biologicals, part of Bio-Techne | Catalog # NB100-64774
![ELISA: Granzyme B Antibody (GB11) [NB100-64774]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-GB11-ELISA-NB100-64774-img0001.jpg "ELISA: Granzyme B Antibody (GB11) [NB100-64774]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # GB11
Concentration
1 mg/ml
Product Specifications
Immunogen
Purified human Granzyme B
Reactivity Notes
Predicted cross-reactivities: Chimpanzee, Monkey, Rhesus Monkey
Specificity
NB100-64774 recognizes the serine protease Granzyme B (GZMB)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Granzyme B Antibody (GB11)
ELISA: Granzyme B Antibody (GB11) [NB100-64774]
ELISA: Granzyme B Antibody (GB11) [NB100-64774] - Analysis of human granzyme B using Mouse anti Human granzyme B as a capture reagent and biotinylated Mouse anti Human granzyme B as a detection reagent with recombinant human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm. Human plasma samples are diluted i:2 (green) and 1:4 (blue).Applications for Granzyme B Antibody (GB11)
Application
Recommended Usage
ELISA
2 ug/ml-5 ug/ml
Flow Cytometry
1:10-1:100
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
For Flow Cytometry: Membrane permeabilisation is required for this application. Use 10 ul of the suggested working dilution to label 10^6 cells or 100 ul whole blood. In ELISA, this product has been used as a capture antibody with NB100-62617 used as the detection antibody.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
Tris buffered saline
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Granzyme B
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Entrez Gene IDs
3002 (Human)
Gene Symbol
GZMB
UniProt
Additional Granzyme B Products
Product Documents for Granzyme B Antibody (GB11)
Product Specific Notices for Granzyme B Antibody (GB11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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