Granzyme B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88151
![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP1-88151-img0011.jpg "Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]")
![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP1-88151-img0014.jpg "Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]")
Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TRAVQPLRLPSNKAQVKPGQTCSVAGWGQTAPLGKHSHTLQEVKMTVQEDRKCESDLRHYYDSTIELCVGDPEIKKTSFKGDSGGPLVCNKVAQGIVSYGRNNGMPPRACTKVSS
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Granzyme B Antibody
![Western Blot: Granzyme B Antibody [NBP1-88151]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-Western-Blot-NBP1-88151-img0009.jpg "Western Blot: Granzyme B Antibody [NBP1-88151]")
Western Blot: Granzyme B Antibody [NBP1-88151]
Western Blot: Granzyme B Antibody [NBP1-88151] - Analysis in control (vector only transfected HEK293T lysate) and GZMB over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP1-88151-img0010.jpg "Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]")
Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]
Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151] - Staining of human liver shows no positivity in hepatocytes as expected.![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP1-88151-img0012.jpg "Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]")
![Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]](https://resources.bio-techne.com/images/products/Granzyme-B-Antibody-Immunohistochemistry-Paraffin-NBP1-88151-img0013.jpg "Immunohistochemistry-Paraffin: Granzyme B Antibody [NBP1-88151]")
Applications for Granzyme B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. ICC/IF reactivity reported in scientific literature (PMID: 22198309).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Granzyme B
Granzyme family serine proteases are stored in the granules of cytotoxic T lymphocytes and natural killer cells. Granzymes contain one S1 protease domain and are synthesized as preproproteins. Granzymes are released toward pathogen infected or transformed target cells during cellular immune reactions. Target cell entry by granzymes is through perforin channels. Granzymes trigger apoptosis by caspase-dependent and -independent mechanisms. There are five granzymes (A, B, G, H and K) in human and many more in mouse and rat.
Alternate Names
CGL-1, CGL1, CSPB, CTLA-1, CTLA1, CTSGL1, Fragmentin-2, Granzyme-2, GRB, GrzB, GZMB, HLP, SECT
Gene Symbol
GZMB
Additional Granzyme B Products
Product Documents for Granzyme B Antibody
Product Specific Notices for Granzyme B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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