GRAP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-805

Novus Biologicals, part of Bio-Techne
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NB100-805
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Bovine (100%), Canine (100%), Mouse (100%), Porcine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all GRAP2 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-GLFPANYVAPMTR corresponding to C-Terminus according to NP_004801.1.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for GRAP2 Antibody

Western Blot: GRAP2 Antibody [NB100-805]

Western Blot: GRAP2 Antibody [NB100-805]

Western Blot: GRAP2 Antibody [NB100-805] - HEK293 overexpressing GRAP2 (RC206546) and probed with GRAP2 (mock transfection in first lane).
Western Blot: GRAP2 Antibody [NB100-805]

Western Blot: GRAP2 Antibody [NB100-805]

Western Blot: GRAP2 Antibody [NB100-805] - staining of Jurkat lysate (30ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

Applications for GRAP2 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:32000

Western Blot

1:100-1:2000
Application Notes
WB: Approx. 38 kDa band observed Jurkat lysates (calculated MW of 37.9 kDa band according to NP_004801.1). In transfected HEK293 transiently expressing GRAP2 a band of approx. 40 kDa is observed. This band is not observed in the non-transfected HEK293.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRAP2

This gene encodes a member of the GRB2/Sem5/Drk family. This member is an adaptor-like protein involved in leukocyte-specific protein-tyrosine kinase signaling. Like its related family member, GRB2-related adaptor protein (GRAP), this protein contains an SH2 domain flanked by two SH3 domains. This protein interacts with other proteins, such as GRB2-associated binding protein 1 (GAB1) and the SLP-76 leukocyte protein (LCP2), through its SH3 domains. Transcript variants utilizing alternative polyA sites exist.

Long Name

GRB2-related Adaptor Protein 2

Alternate Names

GADS, GRB2L, GRBLG, GRBX, Grf40, GRID, GRPL, Mona

Entrez Gene IDs

9402 (Human); 17444 (Mouse)

Gene Symbol

GRAP2

UniProt

NP_004801. (Human)

Additional GRAP2 Products

Product Documents for GRAP2 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GRAP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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