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GRASP55 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38244

Novus Biologicals, part of Bio-Techne
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NBP2-38244
NBP2-38244-25ul

Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: SGELLSSLPPTSNAPSDPATTTAKADAASSLTVDVTPPTAKAPTTVEDRVGDSTPVSEKPVSAAVDANASESP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRASP55 Antibody

Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP2-38244]

Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP2-38244]

Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP2-38244] - Immunohistochemistry analysis in human epididymis and skeletal muscle tissues. Corresponding RNA-seq data are presented for the same tissues.
Western Blot: GRASP55 Antibody [NBP2-38244]

Western Blot: GRASP55 Antibody [NBP2-38244]

Western Blot: GRASP55 Antibody [NBP2-38244] - Analysis in U-251MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GORASP2 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP2-38244]

Immunocytochemistry/ Immunofluorescence: GRASP55 Antibody [NBP2-38244]

Immunocytochemistry/Immunofluorescence: GRASP55 Antibody [NBP2-38244] - Staining of human cell line A-431 shows localization to the Golgi apparatus. Antibody staining is shown in green.

Applications for GRASP55 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRASP55

The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is involved in establishing the stacked structure of the Golgi apparatus. [provided by RefSeq]

Alternate Names

Golgi phosphoprotein 6, golgi reassembly stacking protein 2, 55 kDa, golgi reassembly stacking protein 2, 55kDa, Golgi reassembly-stacking protein of 55 kDa, GOLPH6DKFZp434D156, GRASP55FLJ13139, GRS2Golgi reassembly-stacking protein 2, p59

Gene Symbol

GORASP2

UniProt

Additional GRASP55 Products

Product Documents for GRASP55 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GRASP55 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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