GRB2 Antibody (6E1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15167

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15167
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 6E1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GRB2 Antibodies »

Product Summary for GRB2 Antibody (6E1)

Immunogen

A synthesized peptide derived from human GRB2. UniProt ID (P62993).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRB2 Antibody (6E1)

Western Blot: GRB2 Antibody (6E1) [NBP3-15167]

Western Blot: GRB2 Antibody (6E1) [NBP3-15167]

Western Blot: GRB2 Antibody (6E1) [NBP3-15167] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Mouse Brain whole cell lysate, Rat Brain whole cell lysate. All lanes: GRB2 antibody at 1:1000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 26, 21 KDa. Observed band size: 28 KDa.
Immunohistochemistry: GRB2 Antibody (6E1) [NBP3-15167]

Immunohistochemistry: GRB2 Antibody (6E1) [NBP3-15167]

Immunohistochemistry: GRB2 Antibody (6E1) [NBP3-15167] - IHC image of GRB2 antibody diluted at 1:100 and staining in paraffin-embedded human ovarian cancer. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Applications for GRB2 Antibody (6E1)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: GRB2

The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).

Long Name

Growth Factor Receptor-Bound Protein 2

Alternate Names

ASH, EGFRBP-GRB2, Grb3-3

Gene Symbol

GRB2

Additional GRB2 Products

Product Documents for GRB2 Antibody (6E1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRB2 Antibody (6E1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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