GRB2 Antibody (9K6H0)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15638

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15638-100ul
NBP3-15638-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9K6H0

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GRB2 Antibodies »

Product Summary for GRB2 Antibody (9K6H0)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 118-217 of human GRB2 (P62993). YFLWVVKFNSLNELVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWKGACHGQTGMFPRNYVTPVNRNV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRB2 Antibody (9K6H0)

Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638]

Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638]

Western Blot: GRB2 Antibody (9K6H0) [NBP3-15638] - Analysis of extracts of various cell lines, using GRB2 antibody (NBP3-15638) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (9K6H0) [NBP3-15638]

Immunocytochemistry/ Immunofluorescence: GRB2 Antibody (9K6H0) [NBP3-15638]

Immunocytochemistry/Immunofluorescence: GRB2 Antibody (9K6H0) [NBP3-15638] - Immunofluorescence analysis of HeLa cells using GRB2 Rabbit mAb (NBP3-15638) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: GRB2 Antibody (9K6H0) [NBP3-15638]

Immunohistochemistry-Paraffin: GRB2 Antibody (9K6H0) [NBP3-15638]

Immunohistochemistry-Paraffin: GRB2 Antibody (9K6H0) [NBP3-15638] - Mouse spleen using GRB2 Rabbit mAb (NBP3-15638) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for GRB2 Antibody (9K6H0)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunoprecipitation

1:500 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRB2

The growth factor bound protein 2 (GRB2) is a 25 kDa cytosolic protein that contains one src homology (SH2) domain and two SH3 domains (1). GRB2 associates with Tyrosine phosphorylated EGFRs and PDGFRs via its SH2 domain. GRB2 also plays a crucial role in two separate insulin pathways leading from the insulin receptor to the Ras protein thus affecting mitogenic signaling (2). Additionally GBR2, a bifunctional adaptor protein, recruits Sos for Ras activation and subsequent signal transmission (3).

Long Name

Growth Factor Receptor-Bound Protein 2

Alternate Names

ASH, EGFRBP-GRB2, Grb3-3

Gene Symbol

GRB2

Additional GRB2 Products

Product Documents for GRB2 Antibody (9K6H0)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for GRB2 Antibody (9K6H0)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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