Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2F5

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all GRF2 Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human RAPGEF1(NP_941372) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

122.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRF2 Antibody (OTI2F5) - Azide and BSA Free

Western Blot: GRF2 Antibody (OTI2F5)Azide and BSA Free [NBP2-70846]

Western Blot: GRF2 Antibody (OTI2F5)Azide and BSA Free [NBP2-70846]

Western Blot: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Immunocytochemistry/ Immunofluorescence: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846]

Immunocytochemistry/ Immunofluorescence: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846]

Immunocytochemistry/Immunofluorescence: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY GRF2.
Immunohistochemistry: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846]

Immunohistochemistry: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846]

Immunohistochemistry: GRF2 Antibody (OTI2F5) - Azide and BSA Free [NBP2-70846] - Analysis of Human thyroid tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for GRF2 Antibody (OTI2F5) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:400-4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRF2

GRF2 encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Alternate Names

C3GGRF2guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene), CRK SH3-binding GNRP, DKFZp781P1719, Guanine nucleotide-releasing factor 2, Protein C3G, Rap guanine nucleotide exchange factor (GEF) 1, rap guanine nucleotide exchange factor 1

Gene Symbol

RAPGEF1

Additional GRF2 Products

Product Documents for GRF2 Antibody (OTI2F5) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRF2 Antibody (OTI2F5) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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