GRF2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-06500

Novus Biologicals, part of Bio-Techne
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NBP1-06500
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all GRF2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human CRK SH3-binding GNRP using the numbering given in entry NP_005303.2 (GeneID 2889).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRF2 Antibody

Western Blot: GRF2 Antibody [NBP1-06500]

Western Blot: GRF2 Antibody [NBP1-06500]

Western Blot: GRF2 Antibody [NBP1-06500] - Detection of Human C3G by Western Blot. Samples: Whole cell lysate (5, 15 and 50 ug) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-C3G antibody NBP1-06500 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Western Blot: GRF2 Antibody [NBP1-06500]

Western Blot: GRF2 Antibody [NBP1-06500]

Western Blot: GRF2 Antibody [NBP1-06500] - Whole cell lysate from HeLa cells. C3G was also immunoprecipitated by rabbit anti-C3G antibody NBP1-06501.
Immunoprecipitation: GRF2 Antibody [NBP1-06500]

Immunoprecipitation: GRF2 Antibody [NBP1-06500]

Immunoprecipitation: GRF2 Antibody [NBP1-06500] - Detection of human C3G by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-C3G antibody NBP1-06500 used for IP at 6 ug per reaction. C3G was also immunoprecipitated by rabbit anti-C3G antibody NBP1-06501. For blotting immunoprecipitated C3G, NBP1-06500 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for GRF2 Antibody

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: GRF2

This gene encodes a human guanine nucleotide exchange factor. It transduces signals from CRK by binding the SH3 domain of CRK, and activating several members of the Ras family of GTPases. This signaling cascade that may be involved in apoptosis, integrin-mediated signal transduction, and cell transformation. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some variants has not been determined. [provided by RefSeq]

Alternate Names

C3GGRF2guanine nucleotide-releasing factor 2 (specific for crk proto-oncogene), CRK SH3-binding GNRP, DKFZp781P1719, Guanine nucleotide-releasing factor 2, Protein C3G, Rap guanine nucleotide exchange factor (GEF) 1, rap guanine nucleotide exchange factor 1

Entrez Gene IDs

2889 (Human)

Gene Symbol

RAPGEF1

UniProt

NP_005303.2 (Human)

Additional GRF2 Products

Product Documents for GRF2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRF2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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