GRK2 Antibody (3F8) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37611
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Monkey, Primate
Cited:
Human
Applications
Validated:
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 3F8
Format
BSA Free
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Product Specifications
Immunogen
Purified recombinant fragment of human GRK2 expressed in E. Coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GRK2 Antibody (3F8) - BSA Free
Immunohistochemistry: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]
Immunohistochemistry: GRK2 Antibody (3F8) [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2) in two patients with takotsubo syndrome who underwent endomyocardial biopsy in both the acute and recovery phases. Micrographs showing immunofluorescence stainings of GRK2 in the acute and recovery phases. Arrowheads (yellow) indicate positive signals for GRK2. Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-31034-z) licensed under a CC-BY license.Western Blot: GRK2 Antibody (3F8)BSA Free [NBP2-37611]
Western Blot: GRK2 Antibody (3F8) [NBP2-37611] - Analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611]
Immunocytochemistry/Immunofluorescence: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.Applications for GRK2 Antibody (3F8) - BSA Free
Application
Recommended Usage
ELISA
1:10000
Immunocytochemistry/ Immunofluorescence
1:200 - 1:1000
Immunohistochemistry
1:200 - 1:1000
Immunohistochemistry-Paraffin
1:200 - 1:1000
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Unpurified
Formulation
Ascites
Format
BSA Free
Preservative
0.03% Sodium Azide
Concentration
This product is unpurified. The exact concentration of antibody is not quantifiable.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GRK2
Long Name
G protein-coupled Receptor Kinase 2
Alternate Names
ADRBK1, BARK1, Beta-ARK1
Gene Symbol
GRK2
Additional GRK2 Products
Product Documents for GRK2 Antibody (3F8) - BSA Free
Product Specific Notices for GRK2 Antibody (3F8) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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