GRP75/HSPA9B/Mortalin Antibody (30A5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97448

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Drosophila, Guinea Pig, Hamster, Primate, Rabbit, Sheep, Xenopus

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 30A5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GRP75/HSPA9B Antibodies »

Product Specifications

Immunogen

Native human Grp75 (Mortalin).

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Marker

Immortalization Marker

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for GRP75/HSPA9B/Mortalin Antibody (30A5)

Western Blot: GRP75/HSPA9B/Mortalin Antibody (30A5) [NBP1-97448]

Western Blot: GRP75/HSPA9B/Mortalin Antibody (30A5) [NBP1-97448]

Western Blot: Mortalin Antibody (30A5) [NBP1-97448] - Analysis of Grp75/Mortalin, mAb (30A5): Lane 1: MW marker; Lane 2: HeLa (heat shocked); Lane 3: L-929 (heat shocked); Lane 4: CHO -K1 (heat shocked); Lane 5: GPC-16 (heat shocked); Lane 6: PC-12 (heat shocked).
Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (30A5) [NBP1-97448]

Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (30A5) [NBP1-97448]

Immunohistochemistry: Mortalin Antibody (30A5) [NBP1-97448] - Analysis of human breast cancer tissue immunohistochemically stained using Grp75 mAb (30A5).

Applications for GRP75/HSPA9B/Mortalin Antibody (30A5)

Application
Recommended Usage

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:1000
Application Notes
This antibody is useful in Immunohistochemistry and Western Blot (Detects a band of ~75kDa).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Ammonium sulfate precipitation

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRP75/HSPA9B

Grp75 (Glucose regulated 75 kDa protein) is a member of the Hsp70 family. It is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the re-folding of proteins translocated into this organelle. Like its E. coli homolog DnaK, Grp75 possesses a cation-dependent ATPase activity thought to be central to its function as a chaperone.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

CSA, HSPA9, HSPA9B, Mortalin-2, MOT2, PBP74

Gene Symbol

HSPA9

Additional GRP75/HSPA9B Products

Product Documents for GRP75/HSPA9B/Mortalin Antibody (30A5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRP75/HSPA9B/Mortalin Antibody (30A5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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