GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-70963

Novus Biologicals, part of Bio-Techne
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NBP2-70963
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI9F8

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all GRP75/HSPA9B Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

73.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free

Western Blot: GRP75/HSPA9B/Mortalin Antibody (OTI9F8)Azide and BSA Free [NBP2-70963]

Western Blot: GRP75/HSPA9B/Mortalin Antibody (OTI9F8)Azide and BSA Free [NBP2-70963]

Western Blot: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRP75/HSPA9B/Mortalin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti
Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963]

Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963]

Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GRP75/HSPA9B/Mortalin.
Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963]

Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963]

Immunohistochemistry: GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free [NBP2-70963] - Staining of paraffin-embedded Human endometrium tissue using anti-GRP75/HSPA9B/Mortalin mouse monoclonal antibody.
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Applications for GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50

Immunohistochemistry

1:50

Western Blot

1:1000-2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: GRP75/HSPA9B

The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones facilitating the assembly of multi-protein complexes, participate in the translocation of polypeptides across cell membranes and to the nucleus, and aid in the proper folding of nascent polypeptide chains. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. HSP 70 and HSC 70, which are found in both the cytosol and nucleus of mammalian cells, play key roles in the cytosolic endoplasmic reticulum and mitochondrial import machinery. They are involved in chaperoning nascent polypeptide chains and in protecting cells against the accumulation of improperly folded proteins. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 78 is localized in the endoplasmic reticulum, where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains. Research indicates that members of the HSP 70 family may act as force-generating motors, relying on the hydrolysis of ATP for their activity.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

CSA, HSPA9, HSPA9B, Mortalin-2, MOT2, PBP74

Gene Symbol

HSPA9

Additional GRP75/HSPA9B Products

Product Documents for GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRP75/HSPA9B/Mortalin Antibody (OTI9F8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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