GRP75/HSPA9B/Mortalin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-31596
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat, Porcine
Predicted:
Xenopus (94%). Backed by our 100% Guarantee.
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human GRP75/HSPA9B/Mortalin. The exact sequence is proprietary.
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Zebrafish (87%).
Localization
Mitochondrion
Marker
Immortalization Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
74 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GRP75/HSPA9B/Mortalin Antibody
Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596]
Western Blot: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Grp75 antibody diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596]
Immunocytochemistry/Immunofluorescence: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Grp75 stained by Grp75 antibody diluted at 1:500. Blue: Hoechst 33342 staining.Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596]
Immunohistochemistry-Paraffin: GRP75/HSPA9B/Mortalin Antibody [NBP1-31596] - Rat duodenum. Grp75 antibody diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.Applications for GRP75/HSPA9B/Mortalin Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunohistochemistry-Paraffin
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
PBS (pH 7), 20% Glycerol, 1% BSA
Preservative
0.025% Proclin 300
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GRP75/HSPA9B
Long Name
75 KD Glucose Regulated Protein
Alternate Names
CSA, HSPA9, HSPA9B, Mortalin-2, MOT2, PBP74
Gene Symbol
HSPA9
Additional GRP75/HSPA9B Products
Product Documents for GRP75/HSPA9B/Mortalin Antibody
Product Specific Notices for GRP75/HSPA9B/Mortalin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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