GRP78/HSPA5 Antibody (1H11-1H7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-12888
![Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888]](https://resources.bio-techne.com/images/products/GRP78-HSPA5-Antibody-1H11-1H7-Western-Blot-NBP2-12888-img0003.jpg "Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Bovine, Fungi, Hamster, Monkey, Rabbit, Xenopus
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 1H11-1H7
Concentration
1 mg/ml
Product Specifications
Immunogen
His-tagged human GRP78
Localization
Endoplasmic Reticulum , Endoplasmic Reticulum Lumen , Melanosome
Specificity
Detects a protein with a mass of approx. 78kDa corresponding to GRP78
Marker
ER Stress Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for GRP78/HSPA5 Antibody (1H11-1H7)
Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888]
Western Blot: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - analysis of Human cell lysates showing detection of GRP78 protein using Mouse Anti-GRP78 Monoclonal Antibody, Clone 1H11-1H7 . Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:1000.![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888]](https://resources.bio-techne.com/images/products/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0004.jpg "Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888]")
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888]
Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Magnification: 100x.![Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888]](https://resources.bio-techne.com/images/products/GRP78-HSPA5-Antibody-1H11-1H7-Immunocytochemistry-Immunofluorescence-NBP2-12888-img0005.jpg "Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888]")
Immunocytochemistry/ Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888]
Immunocytochemistry/Immunofluorescence: GRP78/HSPA5 Antibody (1H11-1H7) [NBP2-12888] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-GRP78 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Melanosome. Cytoplasm. Magnification: 20x.Applications for GRP78/HSPA5 Antibody (1H11-1H7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:2000
Application Notes
0.5 ug/ml of GRP78 Antibody was sufficient for detection of Grp78 in 10 ug of rat tissue lysate by ECL immunoblot analysis.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS (pH 7.4), 50% Glycerol
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GRP78/HSPA5
Long Name
75 KD Glucose Regulated Protein
Alternate Names
BIP, HSP70-5, HSPA5
Gene Symbol
HSPA5
Additional GRP78/HSPA5 Products
Product Documents for GRP78/HSPA5 Antibody (1H11-1H7)
Product Specific Notices for GRP78/HSPA5 Antibody (1H11-1H7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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