GRP78/HSPA5 Antibody (A5-C9-G6-R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-32409
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 Clone # A5-C9-G6-R
Concentration
1 mg/ml
Product Specifications
Immunogen
Synthetic peptide within Human GRP78 aa 1-50 / 654. (Uniprot: P11021)
Localization
Endoplasmic reticulum lumen. Melanosome. Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for GRP78/HSPA5 Antibody (A5-C9-G6-R)
Western Blot: GRP78/HSPA5 Antibody (A5-C9-G6-R) [NBP3-32409]
Western Blot: GRP78/HSPA5 Antibody (A5-C9-G6-R) [NBP3-32409] - Western blot analysis of GRP78/HSPA5 on different lysates with Mouse anti-GRP78/HSPA5 antibody (NBP3-32409) at 1/2,000 dilution.Lane 1: HeLa cell lysate (20 ug/Lane)
Lane 2: HepG2 cell lysate (20 ug/Lane)
Lane 3: NIH/3T3 cell lysate (20 ug/Lane)
Lane 4: MCF7 cell lysate (20 ug/Lane)
Lane 5: Mouse testis tissue lysate (40 ug/Lane)
Lane 6: Rat liver tissue lysate (40 ug/Lane)
Predicted band size: 72 kDa
Observed band size: 70/72 kDa
Exposure time: 1 minute 2 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32409) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.
Applications for GRP78/HSPA5 Antibody (A5-C9-G6-R)
Application
Recommended Usage
Western Blot
1:2000
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH7.4), 0.1% BSA and 40% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: GRP78/HSPA5
Long Name
75 KD Glucose Regulated Protein
Alternate Names
BIP, HSP70-5, HSPA5
Gene Symbol
HSPA5
Additional GRP78/HSPA5 Products
Product Documents for GRP78/HSPA5 Antibody (A5-C9-G6-R)
Product Specific Notices for GRP78/HSPA5 Antibody (A5-C9-G6-R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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