GRP78/HSPA5 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB300-520CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB300-520AF350

Alexa Fluor 405

NB300-520AF405

Alexa Fluor 488

NB300-520AF488

Alexa Fluor 532

NB300-520AF532

Alexa Fluor 594

NB300-520AF594

Alexa Fluor 647

NB300-520AF647

Alexa Fluor 700

NB300-520AF700

Alexa Fluor 750

NB300-520AF750

Allophycocyanin

NB300-520APC

Biotin

NB300-520B

DyLight 350

NB300-520UV

DyLight 405

NB300-520V

DyLight 488

NB300-520G

DyLight 550

NB300-520R

DyLight 594

NB300-520DL594

DyLight 650

NB300-520C

DyLight 680

NB300-520FR

DyLight 755

NB300-520IR

FITC

NB300-520F

HRP

NB300-520H

Janelia Fluor 525

NB300-520JF525

Janelia Fluor 549

NB300-520JF549

Janelia Fluor 585

NB300-520JF585

Janelia Fluor 635

NB300-520JF635

Janelia Fluor 646

NB300-520JF646

Janelia Fluor 669

NB300-520JF669

mFluor Violet 450 SE

NB300-520MFV450

mFluor Violet 500 SE

NB300-520MFV500

mFluor Violet 610 SE

NB300-520MFV610

PE

NB300-520PE

PerCP

NB300-520PCP

Forumulation

Catalog #

BSA Free

NB300-520

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Primate (Negative)

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GRP78/HSPA5 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal portion of rat GRP78 (within residues 600-654).

Reactivity Notes

Use in Rat reported in scientific literature (PMID:34519641). Human reactivity reported in scientific literature (PMID: 26865610). Does not cross-react with African Green Monkey (COS cells). Mouse reactivity reported in scientific literature (PMID:32973043).

Marker

ER Stress Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for GRP78/HSPA5 Antibody [CoraFluor™ 1]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: GRP78/HSPA5

Within the ER, misfolded proteins are detected by Bip (GPR78). In the presence of misfolded proteins, GPR78 disscociates from PERK, allowing it to homodimerize and autophosphoyate. In its dimerized phosphorylated form, PERK phosphorylates eIF2. The phosphorylation of eIF2 blocks the majority of translation to prevent the continued accumulation of protein in the ER. GRP78 also binds to IRE1 and ATF6 under normal ER conditions, but dissociates upon accumulation of misfolded proteins. Unbound ATF6 is translocated to the gogli where it is converted into a cleaved, active form. The cleaved form of ATF6 is then able to up regulate transcription of UPR genes including XBP1. Activated IRE1 acts as an endoribonuclease to an intron from XBP1 transcripts. The XBP1 splice variant codes for an active transcription factor which activates transcription of P58IPK. P58IPK is a HSP40 protein which binds to and inhibits PERK. Thus, if the accumulated misfolded proteins have been removed, P58IPK acts to shut down the UPR by removing the block to translation.

Long Name

75 KD Glucose Regulated Protein

Alternate Names

BIP, HSP70-5, HSPA5

Gene Symbol

HSPA5

Additional GRP78/HSPA5 Products

Product Documents for GRP78/HSPA5 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for GRP78/HSPA5 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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