GRSF1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38341

Novus Biologicals, part of Bio-Techne
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NBP2-38341
NBP2-38341-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all GRSF1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: YSQESKTTYLEDLPPPPEYELAPSKLEEEVDDVFLIRAQGLPWSCTMEDVLNFFSDCRIRNGEN

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (88%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GRSF1 Antibody

Western Blot: GRSF1 Antibody [NBP2-38341]

Western Blot: GRSF1 Antibody [NBP2-38341]

Western Blot: GRSF1 Antibody [NBP2-38341] - Analysis using Anti-GRSF1 antibody NBP2-38341 (A) shows similar pattern to independent antibody NBP1-89488 (B).
Immunocytochemistry/ Immunofluorescence: GRSF1 Antibody [NBP2-38341]

Immunocytochemistry/ Immunofluorescence: GRSF1 Antibody [NBP2-38341]

Immunocytochemistry/Immunofluorescence: GRSF1 Antibody [NBP2-38341] - Staining of human cell line U-251 MG shows localization to mitochondria.
Immunohistochemistry-Paraffin: GRSF1 Antibody [NBP2-38341]

Immunohistochemistry-Paraffin: GRSF1 Antibody [NBP2-38341]

Immunohistochemistry-Paraffin: GRSF1 Antibody [NBP2-38341] - Staining of human adrenal gland, colon, lymph node and pancreas using Anti-GRSF1 antibody NBP2-38341 (A) shows similar protein distribution across tissues to independent antibody NBP1-89488 (B).
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Applications for GRSF1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GRSF1

The GRSF1 gene encodes a sequence factor 1 protein that plays a role in binding RNAs that contain the 14 base G-rich element. This protein is located in the cytoplasm and encourages transmission of viral mRNAs in vitro. It codes for two isoforms: Isoform 1 is 480 amino acids long at 53 kDA while isoform 2 is 318 amino acids long at a mass of approximately 36 kDA. The GRSF1 gene interacts with the ICT1, FTSJ1, APC, ULK2, and GABARAP genes. Research on influenza and neuronitis have explored the role of GRSF1 in the development of these diseases.

Alternate Names

FLJ13125, G-rich RNA sequence binding factor 1, G-rich sequence factor 1, GRSF-1

Gene Symbol

GRSF1

UniProt

Q12849 (Human)

Additional GRSF1 Products

Product Documents for GRSF1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GRSF1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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