GSDMDC1 Antibody (5U7R7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16070
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5U7R7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 346-484 of human GSDMDC1 (NP_079012.3). DGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for GSDMDC1 Antibody (5U7R7)
![Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070]](https://resources.bio-techne.com/images/products/GSDMDC1-Antibody-8A8A5-Western-Blot-NBP3-16070-img0004.jpg "Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070]")
Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070]
Western Blot: GSDMDC1 Antibody (8A8A5) [NBP3-16070] - Western blot analysis of extracts of THP-1 cells, using GSDMDC1 antibody (NBP3-16070) at 1:1000 dilution.THP-1 cells were treated by LPS (1 ug/ml) at 37C for 8 hours.THP-1 cells were treated by PMA/TPA (200 nM) at 37C for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.![Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070]](https://resources.bio-techne.com/images/products/GSDMDC1-Antibody-8A8A5-Western-Blot-NBP3-16070-img0005.jpg "Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070]")
Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070]
Western Blot: GSDMDC1 Antibody (8A8A5) [NBP3-16070] - Western blot analysis of extracts of A-431 cells, using GSDMDC1 antibody (NBP3-16070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Analysis of extracts of THP-1, using GSDMD antibody at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.
Analysis of extracts of THP-1, using GSDMD antibody at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.Applications for GSDMDC1 Antibody (5U7R7)
Application
Recommended Usage
Western Blot
1:500 -1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: GSDMDC1
Long Name
Gasdermin-D
Alternate Names
DFNA5L, FKSG10, GSDMD, hGSDMD-CTD)
Gene Symbol
GSDMD
Additional GSDMDC1 Products
Product Documents for GSDMDC1 Antibody (5U7R7)
Product Specific Notices for GSDMDC1 Antibody (5U7R7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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