GSK-3 alpha/beta Antibody (L5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-29873

Recombinant Monoclonal Antibody.
Novus Biologicals, part of Bio-Techne
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NBP2-29873
NBP2-29873-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # L5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GSK-3 alpha/beta Antibodies »

Product Summary for GSK-3 alpha/beta Antibody (L5)

Immunogen

N-terminal 6His-tagged recombinant full length human GSK3Beta.

Reactivity Notes

Human, Mouse

Specificity

GSK3 alpha and beta46kDa(beta)and51kDa(alpha) weight may be detected.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Relevant Entrez Gene Numbers: NM_019884/NM_002093

Scientific Data Images for GSK-3 alpha/beta Antibody (L5)

Western Blot: GSK-3 alpha/beta Antibody (L5) [NBP2-29873]

Western Blot: GSK-3 alpha/beta Antibody (L5) [NBP2-29873]

Western Blot: GSK3 alpha/beta Antibody [NBP2-29873] - HEK293 cell lysate was probed with anti-GSK3alpha /Beta (1:5,000 dilution). Arrows indicate GSK3alpha (~51kDa) and GSK3Beta (~46kDa).

Applications for GSK-3 alpha/beta Antibody (L5)

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

0.1M Tris-Glycine, pH 7.4, 0.15M NaCl, and 30% glycerol.

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: GSK-3 alpha/beta

Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Long Name

Glycogen Synthase Kinase 3

Alternate Names

DKFZp686D0638, EC 2.7.11, EC 2.7.11.26, glycogen synthase kinase 3 alpha, glycogen synthase kinase-3 alpha, GSK-3 alpha

Gene Symbol

GSK3A

UniProt

P49840

Additional GSK-3 alpha/beta Products

Product Documents for GSK-3 alpha/beta Antibody (L5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GSK-3 alpha/beta Antibody (L5)

Relevant Entrez Gene Numbers: NM_019884/NM_002093

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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