GSK-3 alpha/beta Antibody (S08-8G7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19938

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19938-100ul
NBP3-19938-50ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-8G7

Concentration

0.3 mg/ml

View all GSK-3 alpha/beta Antibodies »

Product Summary for GSK-3 alpha/beta Antibody (S08-8G7)

Immunogen

A synthetic peptide of human GSK-3 alpha/beta (Uniprot # P49840)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GSK-3 alpha/beta Antibody (S08-8G7)

Western Blot: GSK-3 alpha/beta Antibody (S08-8G7) [NBP3-19938]

Western Blot: GSK-3 alpha/beta Antibody (S08-8G7) [NBP3-19938]

Western Blot: GSK-3 alpha/beta Antibody (S08-8G7) [NBP3-19938] - Western blot detection of GSK-3 alpha/beta in Jurkat, Rat Brain, C6, CHO-K1, Hela cell lysates using NBP3-19938 (1:1000 diluted). Predicted band size: 51, 47 kDa. Observed band size: 51, 47kDa.

Applications for GSK-3 alpha/beta Antibody (S08-8G7)

Application
Recommended Usage

Western Blot

1/1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSK-3 alpha/beta

Glycogen Synthase Kinase 3beta (GSK-3beta) is a unique serine/threonine kinase that is inactivated by phosphorylation. In response to insulin binding, PKB/AKT phosphorylates GSK-3beta on serine 9, which prevents GSK-3beta from phosphorylating glycogen synthase. Unphosphorylated glycogen synthase is active and able to synthesize glycogen. GSK-3beta is also unique in that it requires a substrate that has been phosphorylated by a distinct kinase before it can phosphorylate the substrate. This phosphate priming mechanism explains why phosphorylation of serine 9 inactivates GSK-3beta. The phosphorylated serine binds to the GSK-3beta priming phosphate position and prevents binding of alternative substrates. In addition to insulin signaling, GSK-3beta participates in the Wnt signaling pathway, where it forms a complex with axin, beta-catenin and adenomatous polyposis coli (APC) protein. In the presence of Wnts, GSK-3beta is unable to phosphorylate beta-catenin, which leads to stabilization of beta-catenin. The Wnt pathway inactivates GSK-3beta via the proteins, Dishevelled and FRAT, which disrupt the interaction of GSK-3beta with axin, beta-catenin, and APC. Clinically, there is considerable interest in GSK-3beta inhibitors because they may mimic the effect of insulin or reduce the hyperphosphorylation of Tau that is observed in Alzheimer's Disease.

Long Name

Glycogen Synthase Kinase 3

Alternate Names

DKFZp686D0638, EC 2.7.11, EC 2.7.11.26, glycogen synthase kinase 3 alpha, glycogen synthase kinase-3 alpha, GSK-3 alpha

Gene Symbol

GSK3A

Additional GSK-3 alpha/beta Products

Product Documents for GSK-3 alpha/beta Antibody (S08-8G7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for GSK-3 alpha/beta Antibody (S08-8G7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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