GSK-3 beta [p Ser9] Antibody (3A6) - BSA Free

Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] -

Immunohistochemistry: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Image of GSK-3 beta [p Ser9] Antibody (3A6) diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Western Blot: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] -

Western Blot: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Positive Western Blot detected in Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate (treated with Calyculin A or not).
All lanes: GSK-3 beta [p Ser9] Antibody at 0.77ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 46 KDa
Observed band size: 46 KDa

Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] -

Immunocytochemistry/Immunofluorescence: GSK-3 beta [p Ser9] Antibody (3A6) [NBP3-26422] - Staining of Hela cells(treated with 50mM Calyculin A for 30min) with GSK-3 beta [p Ser9] Antibody (3A6) at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG (H+L).