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GSPT1 Antibody (CL13336) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44372

Novus Biologicals, part of Bio-Techne
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NBP3-44372

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # CL13336

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of P15170, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for GSPT1 Antibody (CL13336) - Azide and BSA Free

GSPT1 Antibody (CL13336) - Azide and BSA Free Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP3-44372]

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP3-44372]

Staining of human breast cancer shows strong cytoplasmic positivity in tumor cells.
GSPT1 Antibody (CL13336) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: GSPT1 Antibody [NBP3-44372]

Immunocytochemistry/ Immunofluorescence: GSPT1 Antibody [NBP3-44372]

Staining of A-431 cells using the Anti-GSPT1 monoclonal antibody) .
GSPT1 Antibody (CL13336) - Azide and BSA Free Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP3-44372]

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP3-44372]

Staining of human colorectal cancer shows moderate to strong cytoplasmic positivity in tumor cells.

Applications for GSPT1 Antibody (CL13336) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1 ug/ml
Application Notes
For use with IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/ Immunofluorescence/IF, Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSPT1

GSPT1 is involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates theactivity of ERF1. Involved in regulation of mammalian cell growth

Alternate Names

ERF3A, eRF3aFLJ38048, ETF3A, eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, FLJ39067, G1 to S phase transition 1,551G9.2, G1 to S phase transition protein 1 homolog, GST1

Gene Symbol

GSPT1

Additional GSPT1 Products

Product Documents for GSPT1 Antibody (CL13336) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GSPT1 Antibody (CL13336) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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