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GSPT1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47442

Novus Biologicals, part of Bio-Techne
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NBP2-47442
NBP2-47442-25ul

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: GGAANNHGAGSGAGGRAAPVESSQEEQSLCEGSNSAVS

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for GSPT1 Antibody

Western Blot: GSPT1 Antibody [NBP2-47442]

Western Blot: GSPT1 Antibody [NBP2-47442]

Western Blot: GSPT1 Antibody [NBP2-47442] - Analysis in EFO-21 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-GSPT1 antibody. Remaining relative intensity is presented. Loading control: Anti-PARP1.
Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-47442]

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-47442]

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-47442] - Immunohistochemical staining of human rectum shows moderate to strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-47442]

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-47442]

Immunohistochemistry-Paraffin: GSPT1 Antibody [NBP2-47442] - Staining of human breast cancer shows moderate to strong cytoplasmic positivity in tumor cells.

Applications for GSPT1 Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: GSPT1

GSPT1 is involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates theactivity of ERF1. Involved in regulation of mammalian cell growth

Alternate Names

ERF3A, eRF3aFLJ38048, ETF3A, eukaryotic peptide chain release factor GTP-binding subunit ERF3A, Eukaryotic peptide chain release factor subunit 3a, FLJ39067, G1 to S phase transition 1,551G9.2, G1 to S phase transition protein 1 homolog, GST1

Gene Symbol

GSPT1

Additional GSPT1 Products

Product Documents for GSPT1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for GSPT1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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