Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunohistochemistry, Immunoprecipitation, SDS-Page, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 20C11.H5.E11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all GSTM3 Antibodies »

Product Specifications

Immunogen

This Protein A purified GSTM3 Antibody (20C11.H5.E11) was prepared by repeated immunizations in mice with GSTGSTM3 Protein (Uniprot: P21266)

Reactivity Notes

Cross reactivity from other sources has not been determined

Specificity

Reacts specifically with GSTM3 protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

This product is purified from roller bottle culture by Protein A chromatography followed by extensive dialysis against the buffer stated above

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for GSTM3 Antibody (20C11.H5.E11)

SDS-PAGE: GSTM3 Antibody (20C11.H5.E11) [NBP2-44175]

SDS-PAGE: GSTM3 Antibody (20C11.H5.E11) [NBP2-44175]

SDS-Page: GSTM3 Antibody (20C11.H5.E11) [NBP2-44175] - Lane 1: Non-reduced Mouse anti-GSTM3 Monoclonal Antibody. Lane 2: 3 UL OPAL Pre-stained Marker. Lane 3: Reduced Mouse anti-GSTM3 Monoclonal Antibody. Load: 1 Ug per lane. Predicted/Observed size: Non-reduced at 160 kDa/observed at 180-200 kDa; Reduced at 55, 25 kDa. Non-reduced migrates at slightly higher molecular weight.

Applications for GSTM3 Antibody (20C11.H5.E11)

Application
Recommended Usage

ELISA

1:50000-1:200000

Immunohistochemistry

1:10-1:500

Immunoprecipitation

1:10-1:500

SDS-Page

1:100-1:2000

Western Blot

1:500-1:1000
Application Notes
This product has been tested in ELISA and SDS-Page and is suitable in Western Blot. Suitable for most immunological techniques requiring high titer binding and lot-to-lot consistency. Specific conditions for reactivity should be optimized by the end user.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: GSTM3

GSTM3, also known as Glutathione S-transferase Mu 3, is a 225 amino acid that is approx. 27 kDa; is responsible for conjugation of reduced glutathione to an extensive number of exogenous and endogenous hydrophobic electrophiles, and may be able to regulate uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers. Current research is being performed on over 70 diseases and disorders including laryngeal squamous cell carcinoma, pharyngitis, laryngitis, squamous cell carcinoma, laryngeal cancer, oral cancer, cataract, colorectal cancer, carcinoma, breast cancer, astrocytoma, non-Hodgkin lymphoma, malignant pleural mesothelioma, open-angle glaucoma, Hodgkin's lymphoma, lung cancer, basal cell carcinoma, adult brain tumor, and leukoplakia. This protein has been shown to have interactions with over 70 proteins including RBL2, GSTM2, PAK2, MPG, and TFE3 in pathways such as glutathione metabolism, metabolism of xenobiotics by cytochrome P450, drug metabolism - cytochrome P450, establishment of blood-nerve barrier, and response to estrogen stimulus pathway.

Alternate Names

glutathione S-transferase mu 3 (brain), GST5Mu-3, MGC3310, MGC3704, S-(hydroxyalkyl)glutathione lyase M3

Gene Symbol

GSTM3

UniProt

P21266 (Human)

Additional GSTM3 Products

Product Documents for GSTM3 Antibody (20C11.H5.E11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for GSTM3 Antibody (20C11.H5.E11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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